Journal: The EMBO Journal

Article Title: Jacob‐induced transcriptional inactivation of CREB promotes Aβ‐induced synapse loss in Alzheimer's disease

doi: 10.15252/embj.2022112453

Figure Lengend Snippet: Antibodies, chemicals, kits, recombinant proteins, peptides, and software used in the study.

Article Snippet: Anti‐pCREB (Ser133), rabbit polyclonal , Millipore , Cat.: #06‐519; RRID: AB_310153.

Techniques: Recombinant, Software, In Situ, Virus, Blocking Assay, SYBR Green Assay, Reporter Assay, Isolation, shRNA

Journal: Scientific reports

Article Title: Presynaptic dysregulation of the paraventricular thalamic nucleus causes depression-like behavior.

doi: 10.1038/s41598-019-52984-y

Figure Lengend Snippet: Figure 2. Effect of long-term manipulation of PVT neurons by DREADD on long-term wheel-running activity. (A) Wheel-running activity of the mice with AAV-GFP, AAV-hM3Dq, or AAV-hM4Di. Horizontal axis indicates the date from the implantation of CNO pellet (Day 0). Vertical axis shows the movement of the running wheel per day. The thick lines under the record of wheel running indicate the hypo-activity episodes. (B) The frequency of episodes per month before (4 months) and after (3 months) the CNO pellet implantation. *indicates p < 0.05 by post-hoc Dunn’s Multiple Comparison test following Kruskal-Wallis test. (C) images indicate anti-GFP (green) and anti-pCREB (red) immunostaining and nuclear staining with DAPI (blue) in the mice with AAV-GFP, AAV-hM3Dq, or AAV-hM4Di. Scale bar indicates 100 µm. (D) Number of pCREB- positive cells in the three groups, AAV-GFP, AAV-hM3Dq, or AAV-hM4Di. The AAV-GFP group included two mice housed under the LL condition, because they showed similar pCREB staining with mice under the LD condition. Error bars indicate standard error of mean. * and **indicate p < 0.05 and p < 0.01, respectively.

Article Snippet: As primary antibodies, rabbit polyclonal anti-c-fos antibody (1:5000, PC38, Calbiochem, San Diego, CA, USA), rabbit polyclonal anti-pCREB antibody (1:500, #9198, Cell Signaling Technology, Danvers, MA, USA), and chicken polyclonal anti-GFP antibody (1:500, 3Scientific RepoRtS | (2019) 9:16506 | https://doi.org/10.1038/s41598-019-52984-y #GFP-1020, Aves labs, Tigard, OR, USA) were used.

Techniques: Activity Assay, Comparison, Immunostaining, Staining